Comments on: Foundation For Biomedical Research Advertisement http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/ My Confined Space is an image blog with user submitted pictures of anything and everything, funny, serious or dark. Sat, 21 Nov 2009 05:11:42 -0500 http://wordpress.org/?v=2.8.6 hourly 1 By: Robster http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42377 Robster Mon, 23 Apr 2007 23:04:28 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42377 Suck it still sounds full of it. No animal IRB would have approved the swing thunk method. Either Suck it is a liar or the person who said that it was approved was a liar. An IRB would have required a fast acting sedative, or if time was really important, the guiliotine that they had to go back to. This would have had to have been done in a seperate room to prevent stress in the other animals. No surprise you didn't get anything out of it. Your techniques were awful (if even real). Tiki, you left out the important part... "At 6 h when the response was maximal, mice were killed, the small intestines were removed, and gut weight/body weight ratios were calculated." With intestinal weight taken after 6 hours to test the compound for ability to limit fluid loss, popping rodents would have ruined the experiment. Any discomfort or pain would have been limited by time. With the diseases studied in the linked abstract, they include some major killers of people worldwide. If a few mice are the price we pay to get a good anti cholera, C. diff, and E. coli drug, I'm all for it. Thanks for pointing me to this group. I had seen some of their posters before, and need some snark decorations. Suck it still sounds full of it. No animal IRB would have approved the swing thunk method. Either Suck it is a liar or the person who said that it was approved was a liar. An IRB would have required a fast acting sedative, or if time was really important, the guiliotine that they had to go back to. This would have had to have been done in a seperate room to prevent stress in the other animals. No surprise you didn’t get anything out of it. Your techniques were awful (if even real).

Tiki, you left out the important part… “At 6 h when the response was maximal, mice were killed, the small intestines were removed, and gut weight/body weight ratios were calculated.”

With intestinal weight taken after 6 hours to test the compound for ability to limit fluid loss, popping rodents would have ruined the experiment. Any discomfort or pain would have been limited by time. With the diseases studied in the linked abstract, they include some major killers of people worldwide. If a few mice are the price we pay to get a good anti cholera, C. diff, and E. coli drug, I’m all for it.

Thanks for pointing me to this group. I had seen some of their posters before, and need some snark decorations.

]]> By: Nax http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42352 Nax Mon, 23 Apr 2007 20:28:08 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42352 Stop animal research. Start human. Stop animal research. Start human.

]]> By: Illuminatus http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42347 Illuminatus Mon, 23 Apr 2007 20:12:36 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42347 Lo, hence behold the beauty of Plant Phisiology! Suck it kind of stories is why I´d rather work for <i>Monsatan</i> (Greenpeaceish=Monsanto) than helping walk again assholes without enough self-preservation sense to use their safety belt. Lo, hence behold the beauty of Plant Phisiology!

Suck it kind of stories is why I´d rather work for Monsatan (Greenpeaceish=Monsanto) than helping walk again assholes without enough self-preservation sense to use their safety belt.

]]> By: tiki god http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42343 tiki god Mon, 23 Apr 2007 20:00:34 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42343 <i>Suck it</i> <b> Dude. what the fuck? I love their everyday happenings attitude about it: </b><blockquote>Outbred, inbred, and congenic strains of conventional mice which were ano-rectally occluded with cyanoacrylate ester glue and converted to sealed adult mice (SAM) were given, per os, crude cholera enterotoxin (CT) in 10% NaHCO3"</blockquote> <b>Makes it all seem so sterile and cool. Until you have exploding mice on your hands that is.</b> Suck it
Dude. what the fuck? I love their everyday happenings attitude about it:

Outbred, inbred, and congenic strains of conventional mice which were ano-rectally occluded with cyanoacrylate ester glue and converted to sealed adult mice (SAM) were given, per os, crude cholera enterotoxin (CT) in 10% NaHCO3″

Makes it all seem so sterile and cool. Until you have exploding mice on your hands that is.

]]> By: Suck it http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42336 Suck it Mon, 23 Apr 2007 19:40:49 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42336 Even better - in talking with my colleagues today about their work on cholera, they use an mouse model where the anuses have been sealed up with superglue so that they don't poop out all the Vibrio. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=264321&tools=bot Even better – in talking with my colleagues today about their work on cholera, they use an mouse model where the anuses have been sealed up with superglue so that they don’t poop out all the Vibrio.

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=264321&tools=bot

]]> By: Suck it http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42334 Suck it Mon, 23 Apr 2007 19:38:22 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42334 We use the fat pads right on top of the testes. Those have the most white adipocytes of anywhere on the rat. Brown fat isn't as insulin responsive. The standard technique was to spin them against a desk, do a spinal dislocation, pinch the skin above the testes and cut open, then down, and use your two thumbs to pop the testes up and out of the opening, allowing cutting of the fat pad. We use the fat pads right on top of the testes. Those have the most white adipocytes of anywhere on the rat. Brown fat isn’t as insulin responsive. The standard technique was to spin them against a desk, do a spinal dislocation, pinch the skin above the testes and cut open, then down, and use your two thumbs to pop the testes up and out of the opening, allowing cutting of the fat pad.

]]> By: ArtDecoDalek http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42327 ArtDecoDalek Mon, 23 Apr 2007 18:03:19 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42327 "Insulin binding parameters have been measured in testicular membranes of streptozotocin diabetic male rats. Insulin binding decrease was ascribed to the well-known depressing effect of diabetes mellitus on circulating luteinizing hormone (LH). Because both LH and insulin receptors are modulated by pituitary LH and because of their reduction in testes of diabetic rats, we conclude that Leydig cell dysfunction is a secondary disorder associated with this complex metabolic condition. PMID: 3909853 [PubMed - indexed for MEDLINE]" “Insulin binding parameters have been measured in testicular membranes of streptozotocin diabetic male rats. Insulin binding decrease was ascribed to the well-known depressing effect of diabetes mellitus on circulating luteinizing hormone (LH). Because both LH and insulin receptors are modulated by pituitary LH and because of their reduction in testes of diabetic rats, we conclude that Leydig cell dysfunction is a secondary disorder associated with this complex metabolic condition.

PMID: 3909853 [PubMed - indexed for MEDLINE]“

]]> By: chad http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42326 chad Mon, 23 Apr 2007 17:39:42 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42326 Say suck it...what for would you need testes if you work on diabetes? You may use lab jargon, but your story is still bulls*t. Say suck it…what for would you need testes if you work on diabetes?
You may use lab jargon, but your story is still bulls*t.

]]> By: Suck it http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42314 Suck it Mon, 23 Apr 2007 16:54:09 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42314 In my old lab that did diabetes research, we used to harvest rat testes first by taking the rats by the tail and whipping/spinning them fast and hard against a bench to stun them (carbon dioxide isn't good against large rodents, plus the experiments were CO2-sensitive). We then used a dull blade to *crump* push down on their neck and sever their spines. Keep in mind that this was all approved by our Animal Review Board, but when the people upstairs complained about the noise of the rats dying, we had to go to the previously approved method, which was a hand-operated two bladed guillotine that we had to put the conscious rats' heads through, then *crump* cut it off. Unsurprisingly, the rats really didn't like this, especially when the smell of blood was in the air and the still alive rats were smelling it. So I've got mixed feelings about animal research. Especially since we didn't get a single publication out of it after going through about a thousand rats. In my old lab that did diabetes research, we used to harvest rat testes first by taking the rats by the tail and whipping/spinning them fast and hard against a bench to stun them (carbon dioxide isn’t good against large rodents, plus the experiments were CO2-sensitive). We then used a dull blade to *crump* push down on their neck and sever their spines. Keep in mind that this was all approved by our Animal Review Board, but when the people upstairs complained about the noise of the rats dying, we had to go to the previously approved method, which was a hand-operated two bladed guillotine that we had to put the conscious rats’ heads through, then *crump* cut it off. Unsurprisingly, the rats really didn’t like this, especially when the smell of blood was in the air and the still alive rats were smelling it.

So I’ve got mixed feelings about animal research. Especially since we didn’t get a single publication out of it after going through about a thousand rats.

]]> By: Robster http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/comment-page-1/#comment-42302 Robster Mon, 23 Apr 2007 15:29:51 +0000 http://www.myconfinedspace.com/2007/04/23/foundation-for-biomedical-research-advertisement/#comment-42302 Awesome. Must have poster. Awesome. Must have poster.

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